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Glucose oxidase detection reagent

A project log for Open source Arduino blood glucose meter shield

Open hardware and software blood glucose meter using electrochemical test strips

m-bindhammerM. Bindhammer 07/25/2016 at 17:493 Comments

If we want to produce our own glucose test strips, we need glucose oxidase. I have still no clue where to buy glucose oxidase or if I will be able to produce it by myself (extracted from the fungus Aspergillus niger?), but I came up with a glucose oxidase detection reagent in the mean time. The idea is as following:

Unfortunately I run out of test strips, so I need to buy more to pursue my idea of a glucose oxidase detection reagent.

Discussions

M. Bindhammer wrote 07/26/2016 at 09:12 point

Thanks, Tom. I ordered 10g over my company.

Edit: Sigma Aldrich alias Merck sucks. Even as a company you need to do a lot of paper work. Will cancel this order.

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Tom Meehan wrote 07/27/2016 at 05:29 point

Looks tougher to find than I thought. If you haven't found it yet try looking at the links from Washington University ( http://libguides.wustl.edu/c.php?g=46849&p=300021  ) and search using the CAS numbers (beta-D-Glucose oxidase CAS# 9051-05-2, Glucose Dehydrogenase CAS# 9028-53-9).  I was not sure from your reply if you found it and ordered or not, so I thought I'd forward more resources if you still needed them.  Good luck!

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Tom Meehan wrote 07/26/2016 at 07:41 point

SigmaAldrich carries it (Fisher Scientific probably carries it also): 

http://www.sigmaaldrich.com/technical-documents/protocols/biology/enzymatic-assay-of-glucose-oxidase.html#M 

Scroll down or click on the tab to the right (Related Links/Product list), either way takes you down to what you can order.  

Many strips also use GDH - glucose dehydrogenase as a reagent.

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